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PROTOCOLS

ORGANOIDS CULTURE AND MICROINJECTION

Methods from Bartfeld and Clevers JoVE 2015

GASTRIC ORGANOIDS

Methods from Bartfeld et al. Gastroenterology 2015

INITIATION OF GASTRIC TUMOR ORGANOIDS

Methods from Wallaschek et al JMB 2019

PRODUCTION OF WNT3A-, RSPO1- OR NOG-CONDITIONED MEDIUM

Methods from Wallaschek et al JMB 2019

ACTIVITY TESTING OF WNT AND RSPO- CONDITIONED MEDIUM

Methods from Wallaschek et al JMB 2019

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